Description: The locus D has two alleles D> d. Color dilution is inherited autosomal recessive, that is, recessive homozygous dd is able to dilute eumelanin (Drogemuller et al., 2007a). Black eumelanin is diluted to metallic blue or also called silver gray. Brown to red eumelanin is diluted to a creamy resulting phenotype called Isabella. To obtain complete information about locus D, we need to know the genotypes at loci E and B. The production of eumelanin or pheomelanin depends on the E allele. black or brown eumelanin. If a dog has the E_B_dd genotype, eumelanin will be diluted and the resulting phenotype will be metallic blue/silver-gray. If the dog has the genotype E_bbdd the resulting phenotype will be a cream color, Isabella.


Inheritance: autosomal recessive


Mutation: c.-22G>A in MLPH gene


Sample: EDTA whole blood (1.0 ml) or 2 buccal brushes. For official purposes, the confirmation of the dog’s identity by Veterinarian is recommended.


The analysis is suitable for the following breeds: Australian Shepherd, Briard, Doberman Pincher, Great Dane, German Pinscher, Labrador Retriever, Large Munsterlander, Miniature Pincher, Newfoundland, Rhodesian Ridgeback,